Using an established larval zebrafish infection model

Using an established larval zebrafish infection model

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Functional studies on thymocytes and splenocytes were carried out in Chapter 3 to determine whether thymocytes were relatively free from contaminating B cells and could be used as a “pure” source of T cells for cellular reconstitution experiments. It was shown that both thymocytes and splenocytes could be stimulated in vitro by the B cell nitrogen Escherichia coli lipopolysaccharide to differentiate into cytoplasmic immunoglobulin M producing cells. Thus thymocytes were found to include a population of B lymphocytes.

steroid The innate immune system is the primary defence against the versatile pathogen, Staphylococcus aureus. How this organism is able to avoid immune killing and cause infections is poorly understood. Using an established larval zebrafish infection model, we have shown that overwhelming infection is due to subversion of phagocytes by staphylococci steroid side effects, allowing bacteria to evade killing and found foci of disease. steroid

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