Functional studies on thymocytes and splenocytes were carried out in Chapter 3 to determine whether thymocytes were relatively free from contaminating B cells and could be used as a “pure” source of T cells for cellular reconstitution experiments. It was shown that both thymocytes and splenocytes could be stimulated in vitro by the B cell nitrogen Escherichia coli lipopolysaccharide to differentiate into cytoplasmic immunoglobulin M producing cells. Thus thymocytes were found to include a population of B lymphocytes.
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